Pyrosequencing is a detection technology based on the principle of sequencing-by-synthesis, which provides quantitative real-time data without the need for gels, probes, or labels. In addition to characterizing single nucleotide polymorphisms (SNPs), insertion-deletions (indels), and unknown sequence variants, it can sensitively quantify allele frequencies and DNA methylation levels at both CpG and non-CpG sites. PyroMark produced by Qiagen in Germany integrates detection and quantification of genetic variation into one powerful system, allowing for analysis of targeted short DNA sequences at single base resolution.

 

 

Key Features of PyroMark Q48:

• De novo sequencing
• CpG/CpN methylation measurement
• Single nucleotide polymorphisms analysis
• Mutation analysis for insertion-deletion
• Microbial ID and Drug Resistance Typing
• 48 samples with 4 sequencing primers per run
• Maximum read length 10-140 bp or more
• Fully automated plate

 

Overall, PyroMark is a powerful short DNA sequencer for quantitative DNA mutation and modification analyses.

 

Ref: https://www.qiagen.com/it/service-and-support/learning-hub/technologies-and-research-topics/pyrosequencing-resource-center/technology-overview/